Can I use
?

FIDO U2F API

- UNOFF

JavaScript API to interact with Universal Second Factor (U2F) devices. This allows users to log into sites more securely using two-factor authentication with a USB dongle.

Chrome

  1. 4 - 37: Not supported
  2. 38 - 40: Not supported
  3. 41 - 97: Supported
  4. 98 - 105: Disabled by default
  5. 106: Not supported
  6. 107 - 109: Not supported

Edge

  1. 12 - 18: Not supported
  2. 79 - 104: Supported
  3. 105: Supported

Safari

  1. 3.1 - 12.1: Not supported
  2. 13 - 15.6: Supported
  3. 16.0: Supported
  4. 16.1 - TP: Supported

Firefox

  1. 2 - 46: Not supported
  2. 47 - 66: Disabled by default
  3. 67 - 104: Supported
  4. 105: Supported
  5. 106 - 107: Supported

Opera

  1. 9 - 39: Not supported
  2. 40: Supported
  3. 41: Not supported
  4. 42 - 90: Supported
  5. 91: Supported

IE

  1. 5.5 - 10: Not supported
  2. 11: Not supported

Chrome for Android

  1. 105: Not supported

Safari on iOS

  1. 3.2 - 13.2: Not supported
  2. 13.3 - 15.6: Supported
  3. 16.0: Supported
  4. 16.1: Supported

Samsung Internet

  1. 4 - 17.0: Not supported
  2. 18.0: Not supported

Opera Mini

  1. all: Not supported

Opera Mobile

  1. 10 - 12.1: Not supported
  2. 64: Not supported

UC Browser for Android

  1. 13.4: Not supported

Android Browser

  1. 2.1 - 4.4.4: Not supported
  2. 105: Not supported

Firefox for Android

  1. 104: Supported

QQ Browser

  1. 13.1: Not supported

Baidu Browser

  1. 13.18: Not supported

KaiOS Browser

  1. 2.5: Disabled by default

The U2F API is being decommissioned (only the API, not the U2F protocol) and superseded by WebAuthn.

Resources:
Mozilla bug
Mozilla Firefox bug to remove the U2F API
Chrome platform status: U2F Security Key API removal (Cryptotoken Component Extension)
Chromium Intent to Deprecate and Remove: U2F API (Cryptotoken)
Google Security article
Yubico blog post about the decommission